Application Note on Metabolomics and Lipidomics

This application note presents a comprehensive study combining bioenergetic analyses with metabolomic and lipidomic analyses to investigate metabolic adaptations in cancer cells treated with tyrosine kinase inhibitors. It outlines the methodologies employed, including the use of the Agilent Seahorse XF Pro analyzer for cellular-resolution monitoring of metabolic flux and the Agilent Revident Q-TOF LC/MS for molecular-resolution monitoring. The study identifies significant metabolic changes in leukemia cells treated with specific inhibitors, revealing that these cells upregulated anaplerosis to compensate for reduced mitochondrial function. The document details the workflow from cell culture to analysis, emphasizing the integration of various Agilent technologies to achieve a multi-faceted understanding of drug-induced metabolic pathway modulation. The findings underscore the potential of this multi-technique approach in uncovering new biological hypotheses and understanding the mechanisms of action of anticancer drugs.