Automated Workflow for Quantifying GLP-1 Analog Tirzepatide

This application note details a technical report on an automated workflow utilizing liquid chromatography/mass spectrometry (LC/MS) for the quantification of the glucagon-like peptide-1 (GLP-1) analog tirzepatide in human plasma. The workflow achieved a lower limit of quantification (LLOQ) of 0.05 ng/mL and demonstrated linearity up to 1,000 ng/mL, indicating a wide dynamic range. The report outlines the materials and methods used, including the specific instruments and software involved in the analysis. It discusses the optimization of sample preparation and LC/MS conditions to enhance sensitivity and reproducibility. The results section presents findings on the calibration curve, precision, and accuracy of the assay, confirming that all quality control samples met the acceptance criteria set by regulatory agencies. The conclusion emphasizes the workflow's efficiency and applicability to other GLP-1 analogs, highlighting its significance in drug discovery and development.