Long-Term Robustness of Agilent LC/Q-TOF Systems for Untargeted Lipidomics

This application note presents a technical report on the performance evaluation of Agilent LC/Q-TOF systems for large-scale untargeted lipidomics analyses. The study involved the analysis of over 14,000 human plasma samples collected from children diagnosed with type 1 diabetes mellitus over a period of 26 months. The objective was to assess the precision and mass accuracy of quantitative measurements, which are critical for minimizing variability in lipidomic workflows. The report details the methodologies employed, including the use of positive and negative electrospray ionization modes, quality control measures, and systematic error removal techniques. The results indicate that the Agilent systems provide reliable data quality, with median relative standard deviations of less than 2% for pool quality control samples. The findings support the applicability of these systems in high-confidence, long-term lipidomic analyses, which are essential for understanding the biological variance in clinical research.