Methodology for Toxicological Drug Screening

This application note is a technical report that details a methodology for toxicological drug screening in complex biological matrices. The method utilizes the Agilent Revident liquid chromatography/quadrupole time-of-flight mass spectrometer (LC/Q-TOF MS) along with the Agilent ChemVista spectral library manager and MassHunter Quantitative Analysis software. The report outlines a complete screening workflow that includes sample preparation, suspect screening, and data analysis results for toxicological drugs. It describes the advantages of using data-independent acquisition (DIA) for screening, which allows for the identification of new compounds and differentiation of coeluting isomers. The document also presents experimental details, including sample preparation techniques for plasma and urine, as well as the specific equipment and methods used in the analysis. Furthermore, it discusses the performance of the LC Screener tool in reviewing results and the management of spectral libraries using ChemVista, emphasizing the importance of accurate identification and data management in forensic toxicology.