Summary
This document is a technical report that outlines the systematic approach taken by Almac for peptide UHPLC method development. It describes the complexities involved in developing analytical methods for peptides compared to small molecules, emphasizing the need to adapt methodologies due to the unique characteristics of peptide structures. The report details a method screening process that allows for the generation of multiple chromatographs efficiently, significantly reducing the time required for preliminary data collection. It explains how Almac collaborates with equipment manufacturers to create a peptide pre-screen that provides a high-level indication of optimal parameters for further method development. Additionally, the document discusses the importance of peak purity and the use of mass spectroscopy in the analysis process. It highlights the expertise of Almac's team of peptide analytical chemists and their experience in developing complex analytical methods, which contributes to the robustness and efficiency of the method development process.
