Automated Workflow for Cytotoxicity Assessment

This technical report outlines the development of an automated workflow designed to determine the mechanism of chemical-mediated cytotoxicity using the CyBio FeliX pipetting system. The report describes the use of in vitro cell-based assays, which are essential for evaluating test compound toxicity in drug discovery research. The CyBio FeliX instrument is employed to automate all liquid handling tasks associated with cytotoxicity assays, thereby enhancing efficiency and consistency. The report details the multiplexing of the CellTox™ Green Assay with either the CellTiter-Glo® 2.0 or Caspase-Glo® 3/7 Assay, which minimizes sample processing. The workflow involves the use of thaw-and-use cells, which simplifies the process by eliminating prior cell culture activities. The report also discusses the instrumentation used, including the GloMax® Discover multimode detection system, and provides a detailed description of the assays and reagents utilized in the profiling process. Overall, this report serves as a comprehensive guide for implementing automated cytotoxicity profiling.