Comparing Liquid and Lyophilized One-Step RT-qPCR Master Mix

This application note serves as a technical report detailing the evaluation of the compatibility of liquid and lyophilized One-Step RT-qPCR master mixes with the qTOWER iris real-time PCR thermal cycler. The objective is to assess the performance attributes of both master mix types in terms of reproducibility, PCR efficiency, and multiplex capabilities. The study outlines the experimental setup, including the use of specific RNA samples and primers, and describes the methodology for assessing key performance parameters. Results indicate that both master mixes yield similar Ct values and PCR efficiencies across various formats, demonstrating their compatibility with the qTOWER iris. The findings suggest that lyophilized master mixes can serve as a reliable alternative to liquid mixes, offering advantages in terms of stability and ease of transport. The report concludes that the qTOWER iris is suitable for efficient amplification regardless of the master mix type used, supporting a wide range of qPCR applications.