Utilizing Multiwavelength Data in DGE-AUC

This application note is a case study focusing on the use of multiwavelength (MWL) absorbance data within the framework of density gradient equilibrium analytical ultracentrifugation (DGE-AUC). It presents data on drug-loaded liposomes, specifically liposomal Doxorubicin, and control liposomes, detailing the analysis conducted using MATLAB scripts provided in the appendix. The document outlines previous installments in the series, which introduced DGE-AUC techniques, physical principles, sample preparation, and data analysis fundamentals. It highlights the challenges and opportunities associated with analyzing liposomes compared to other nanoparticle systems, particularly in terms of stability and detection capabilities. The results section discusses the UV-vis spectra obtained from the experiments, showcasing the differences in absorbance between drug-loaded and control liposomes. Additionally, it describes the experimental parameters and methodologies employed in the DGE-AUC experiments, emphasizing the significance of multiwavelength data for identifying various peaks in the absorbance spectra.