Evaluation of Lentiviral Vector Stability and Ion Exchange Purification Process

This technical report presents the findings of Ghosh et al. regarding the stability of lentiviral vectors (LVVs) and the development of ion exchange chromatography (IEX) purification processes. The study investigates the stability of LVVs under varying conditions such as pH, temperature, and ionic strength, highlighting that the vectors maintain good stability in phosphate buffer at pH 6.5–7.5. The research employs a high-throughput batch screening method to identify optimal resins for LVV recovery, particularly focusing on Macro-Prep High Q Resin, which demonstrated significant recovery rates exceeding 60%. The report details the experimental procedures, including the use of a 96-well plate for resin screening and subsequent evaluations of elution conditions. The findings indicate that the Macro-Prep High Q Resin is effective for LVV purification, achieving over 70% yield while reducing host cell proteins. The report concludes with implications for process design in biopharmaceutical manufacturing, emphasizing the resin's scalability and stability under various conditions.