Evaluation of Lentiviral Vector Stability and Purification Process

This technical report presents the findings of Ghosh et al. regarding the stability of lentiviral vectors (LVVs) and the development of ion exchange purification processes. The study investigates various conditions affecting LVV stability, including pH, temperature, and ionic strength, ultimately determining optimal conditions for screening resins aimed at enhancing LVV purity and integrity. A high-throughput batch screening approach was utilized to identify effective wash and elution steps, leading to a refined process that achieved over 70% yield of infectious LVVs while reducing host cell proteins. The report details the use of Macro-Prep High Q Resin, a strong anion exchange resin, which demonstrated significant advantages in terms of flow rates and binding capacity. The findings support the resin's application in process-scale workflows for LVV purification, emphasizing the importance of pH and ionic strength in optimizing recovery and purity. The report concludes with a discussion of the implications for future lentiviral vector purification methodologies.