Histidine-Tagged Protein Purification for Vaccines

This document is a technical report that outlines the purification process of histidine-tagged proteins, specifically focusing on the receptor binding domain (RBD) of the SARS-CoV-2 spike protein. It discusses the challenges associated with achieving high yield and purity for vaccine production, emphasizing the need for efficient and cost-effective purification methods. The report details the use of Nuvia IMAC Resin, an immobilized metal affinity resin, which has demonstrated effectiveness in purifying recombinant proteins from insect cell-baculovirus systems. The authors, Poodts et al. (2022), describe their approach to enhance the synthesis of His6-tagged SARS-CoV-2 RBD, achieving a yield of 21.1 ± 3.7 mg rRBD per liter of cell culture supernatant with an 82% recovery rate. The findings indicate the resin's applicability in vaccine research and development, highlighting its high dynamic binding capacity and scalability for large-scale production.