Cold Capture Method for High Titer Antibody Clones

This document is a technical report detailing the cold capture method for identifying high titer antibody-producing clones in Chinese hamster ovary (CHO) cells. It outlines the challenges faced in cell line development for monoclonal antibody (mAb) production, particularly the heterogeneity of CHO cells that leads to varied mAb production. The report describes the cold capture technique, which involves cooling CHO cells to enhance the labeling of secreted antibodies on the cell surface, thereby facilitating the identification of high-producing clones. It also compares traditional methods like limiting dilution and flow cytometry (FACS) with the Bio-Techne Pala Cell Sorter and Single Cell Dispenser, which offers advantages in efficiency, ease of use, and reduced cell stress. The document presents findings that demonstrate a significant increase in IgG production when using the cold capture method in conjunction with the Bio-Techne platform, highlighting its potential to improve the efficiency of isolating high-yield clones for mAb production.