Comparison of TcBuster and Lentiviral Gene Transfer Methods

This application note presents a detailed comparison between the TcBuster non-viral gene editing system and traditional lentiviral vectors in the context of CAR-T cell manufacturing. It outlines the challenges associated with lentiviral vectors, such as batch-to-batch variability, long lead times, and high costs, prompting the exploration of non-viral methods. The TcBuster system is described as a stable gene transfer solution that utilizes DNA transposition for efficient integration of genes of interest (GOIs) into host cells. The document details the methodology employed in the study, including the use of electroporation for introducing TcBuster components into T cells, and compares the efficiency of gene transfer, cell viability, and T cell functionality between the two methods. Results indicate that TcBuster not only matches but may outperform lentiviral vectors in several key performance areas, including integration efficiency and T cell phenotype, while also offering a faster time to clinic and reduced costs. Genetic characterization and T cell receptor diversity analysis further support these findings.