Guide to SupersonicIEF Method for icIEF Assays

This document is a guide focused on the SupersonicIEF method for optimizing icIEF assays on Maurice and MauriceFlex systems. It outlines the principles behind the SupersonicIEF method, including the use of ampholytic blockers, stepped voltage ramps, and lower ampholyte concentrations to enhance the speed and efficiency of charge variant analysis. The guide details the specific conditions for sample preparation and running protocols, emphasizing the significant reduction in total run time, achieving results in as little as 5 minutes. Additionally, it presents the benefits of the SupersonicIEF method, such as improved reproducibility and suitability for high-throughput applications. The document also discusses the application of the SupersonicIEF method to fractionation workflows, highlighting optimizations that lead to faster focusing and mobilization times. It provides essential materials and reagents required for the method, along with considerations for sample concentration and its impact on assay performance. Overall, the guide serves as a comprehensive resource for researchers looking to implement the SupersonicIEF method in their workflows.