Advancements in Immunopeptidomics Research at Broad Institute

This technical report discusses the advancements in immunopeptidomics research at the Broad Institute’s Proteomics Platform, led by Dr. Claudia Ctortecka. The document outlines the application of trapped ion mobility spectrometry (TIMS) for enhancing proteomics methodologies. It details the challenges associated with immunopeptidomics, particularly in identifying and quantifying immunopeptides presented by major histocompatibility complex (MHC) molecules. The report describes the innovative approaches taken by Dr. Ctortecka and her team to develop a high-throughput, sensitive mass spectrometry workflow aimed at improving the detection of clinically relevant T cell antigens. The document further explains the importance of collaboration in research, highlighting ongoing projects that leverage advanced proteomics techniques to explore cellular interactions and disease mechanisms. The findings emphasize the significance of optimizing acquisition parameters and employing sophisticated analytical methods to push the boundaries of immunopeptidomics research.