APP96 iST Automation Platform for Proteomic Sample Preparation

This application note presents the APP96 iST automation platform, a guide for high-throughput proteomic sample preparation. It outlines the platform's capabilities in fully automating sample processing for LC-MS-based proteomic analysis, significantly reducing hands-on time and minimizing potential human error. The APP96 system is designed to process up to 96 samples daily, making it suitable for large-scale studies. The document details the hardware configuration, including a robotic arm and various components that facilitate efficient sample handling. It also describes the sample preparation workflow, which integrates iST technology for effective bottom-up proteomics. The study benchmarks the APP96 against traditional manual methods, demonstrating its superior performance in protein identification and reproducibility across different sample types, including yeast, mammalian, and insect cells, as well as human blood plasma. The results indicate that the APP96 platform enhances sensitivity and efficiency, particularly at low protein inputs, making it a valuable tool in diverse proteomic applications.