Corning Matrigel Matrix Handling Tips

This guide provides detailed handling tips for Corning Matrigel matrix, which is widely used in life sciences for over 30 years. It outlines best practices for storage, dilution, and coating surfaces with Matrigel matrix to ensure optimal performance in both 2D and 3D applications. The document specifies that Matrigel matrix should not be stored in frost-free freezers or on freezer doors and recommends aliquoting into freezer-compatible tubes after the first thaw. It details the appropriate storage temperatures and the duration for which frozen Matrigel matrix remains viable. Additionally, it explains the dilution process, emphasizing the importance of protein concentration over standard volume dilution. The guide also describes how to coat surfaces effectively and the necessary conditions for thawing the matrix to maintain its integrity. Overall, the document serves as a comprehensive resource for researchers utilizing Matrigel matrix in their experiments.