Evaluation of Videodrop for Lentiviral Vector Characterization

This technical report evaluates the Videodrop, an optical device developed by Myriade for the characterization of lentiviral vectors in gene and cell therapy applications. The report outlines the growing need for effective bioprocess control and characterization tools in the production of lentiviral vectors, particularly for drug product release and in-process controls. The study compares the performance of the Videodrop, which utilizes Interferometric Light Microscopy (ILM), against established methods such as p24 ELISA, RT-qPCR, and Nanoparticle Tracking Analysis (NTA). Results indicate that the Videodrop provides robust measurements of lentiviral vector concentration and size distribution, demonstrating high correlation coefficients with the other methods. The report details the methodology, including the analysis of various lentiviral vector samples and the assessment of the device's linearity and repeatability. The findings suggest that the Videodrop is a suitable and efficient alternative for monitoring lentiviral vector production, facilitating real-time quality control and process optimization.