Performance Comparison of PET and PETG Media Bottles

This technical report evaluates the performance of Corning PET media bottles compared to Competitor PETG bottles for the storage of serum at low temperatures. The study focuses on the impact of storage conditions on serum integrity and cell culture outcomes. Serum was stored in both types of bottles at -80°C for 30 days, after which it was thawed and used to culture L929 and AE-1 cells. The results indicated no significant differences in serum performance, as measured by cell expansion and morphology, between the two bottle types. The report outlines the materials and methods used, including the specific cell lines and culture conditions. It concludes that Corning PET media bottles are suitable for serum storage at low temperatures, maintaining the serum's integrity and functionality for cell culture applications. This study provides valuable insights for researchers in the life sciences field regarding the selection of storage containers for biological reagents.