Key Advantages of Mass Pooling with truCOVER

This technical note outlines the advantages of using the truCOVER WGS PCR-free Library Prep Kit for whole-genome sequencing (WGS) workflows. It details how the kit enhances operational efficiency by enabling high quantification concordance between Qubit and qPCR, thereby eliminating the need for time-consuming qPCR-based quality control steps. The document presents findings that demonstrate a reduction in preparation and pooling time by up to 30%, along with a significant decrease in overall costs. Additionally, it discusses the kit's ability to deliver robust and reproducible sequencing coverage across diverse sample types, ensuring reliable sequencing metrics. The note also addresses common library preparation challenges, such as inconsistent DNA fragment sizes and variable library quality, and explains how the truCOVER kit mitigates these issues through mechanical shearing. The results indicate that libraries prepared with truCOVER achieve high fidelity in quantification and consistent fragment size distributions, making it a suitable solution for high-throughput laboratories.