Implementing Raman Spectroscopy for Bioprocess Monitoring

This application note presents the implementation of Raman spectroscopy for the automated monitoring of a cell culture bioprocess, specifically focusing on CHO cell fed-batch processes. It outlines the significance of optimizing upstream bioprocessing in the biopharmaceutical industry through real-time data acquisition of process parameters, cells, and products. The document details how Raman spectroscopy serves as a Process Analytical Technology (PAT) to analyze these parameters efficiently. It describes the methodology for acquiring bioprocess data using SciVario twin bioreactor systems equipped with a ProCellics Raman Analyzer. The note emphasizes the importance of monitoring critical parameters such as glucose, lactate, and viable cell density to ensure high-quality production. Additionally, it discusses the chemometric modeling necessary for interpreting Raman spectra and the advantages of real-time measurements for process control. The results indicate that Raman spectroscopy effectively monitored cell culture runs, achieving significant cell densities while providing insights into the bioprocess dynamics.