OptiPRO SFM AGT Medium for Viral Vaccine Production

This application note details the capabilities of Gibco™ OptiPRO™ SFM AGT Medium in supporting viral vaccine production. It compares the performance of the AGT format to the traditional liquid format, highlighting its benefits for larger-scale production. The AGT medium, known for its serum-free and animal origin-free formulation, offers advantages such as faster dissolution, lower dust generation, and a simplified preparation workflow. The document outlines a study evaluating the growth and productivity of Vero cells cultured in both formats, assessing viable cell density, cumulative population doublings, and viral titer production. Results indicate that the AGT format performs comparably to the liquid format across multiple passages, confirming its suitability for consistent and streamlined viral vaccine production. The findings emphasize the importance of selecting an appropriate medium early in development to facilitate scale-up processes. Additionally, the note provides details on the reconstitution process and the performance metrics of both media formats, ensuring reliable supply and product quality.