This guide presents a comprehensive framework for threat hunting within cybersecurity operations. It outlines the increasing importance of threat hunting as organizations face evolving cyber threats and operational risks. The document details the challenges organizations encounter in establishing effective threat hunting capabilities, including skills shortages and technical impediments such as data deficits. It emphasizes the necessity of a proactive and iterative approach to threat hunting, which aims to identify previously undetected threats. The guide also discusses the role of threat hunting in conjunction with other security operations, highlighting its outputs, such as threat detection content creation, indicators of compromise, and enhanced documentation like playbooks. Furthermore, it addresses the prerequisites for successful threat hunting, including technological and personnel requirements. The framework aims to provide organizations with the operational and technical guidance needed to develop sustainable threat hunting practices.