Guidance for Optimisation and Validation of Custom Oligonucleotides

This guide provides detailed instructions for the optimisation and validation of custom oligonucleotides, specifically focusing on their handling in laboratory settings. It outlines the process of characterising oligonucleotides upon receipt, including the importance of documenting key information such as sales order numbers and purification methods. The guide explains the resuspension of oligonucleotides, recommending suitable buffers and concentrations for various applications like PCR and qPCR. It details the calculations required to prepare stock solutions and the methods for measuring oligo concentrations using spectrophotometry. Furthermore, it addresses potential issues with resuspension and offers troubleshooting advice. The document emphasizes the significance of adhering to specific guidelines for measuring oligo concentrations to ensure accuracy and reliability in experimental results. Overall, this guide serves as a comprehensive resource for researchers working with custom oligonucleotides, providing essential protocols and best practices.