Spectroelectrochemistry for Uric Acid Analysis

This Application Note details the use of spectroelectrochemistry as an analytical technique for the determination of uric acid (UA) concentrations. It outlines the methodology employed, which includes obtaining two calibration curves—one from electrochemical data and another from spectroscopic data—within a single set of experiments. The study confirms the effectiveness of this technique by demonstrating that both methods yield comparable results for UA concentration. The instrumentation utilized includes the SPELEC device and DropView SPELEC software, which facilitate the acquisition and analysis of data. The results show that the absorbance at 285 nm increases with UA concentration, and the calibration curves illustrate the relationship between current intensity and absorbance. The autovalidated nature of the method is highlighted, as both calibration curves provide consistent concentration estimates for the tested samples. This document serves as a comprehensive guide for analysts seeking to employ spectroelectrochemistry in quantitative analyses.