This guide provides an introduction to AI literacy, emphasizing the importance of knowledge in making informed decisions regarding AI systems. It outlines resources developed by Microsoft, tailored for individuals with varying levels of AI education and experience. The guide addresses the AI literacy requirements set forth by the EU AI Act, which will be effective from February 2, 2025, and encourages organizations to assess how these requirements apply to their specific contexts. It categorizes AI literacy materials into three levels: fundamentals, intermediate, and advanced, allowing users to select resources based on their experience. The guide includes a variety of educational materials, such as blog posts, webpages, and modular coursework, aimed at enhancing understanding of responsible AI practices. Additionally, it presents resources for both using and developing AI, ensuring accessibility for learners at different stages of their AI literacy journey.