High-Throughput Single-Cell Targeted DNA Sequencing Solutions

This technical report outlines the advancements in single-cell targeted DNA sequencing solutions developed by Mission Bio, specifically utilizing the Tapestri platform. The report describes the platform's capabilities in analyzing genetic variations within cancer, employing droplet microfluidics and a multiplex PCR-based approach. It details updates on catalog cancer panels, including acute myeloid leukemia, chronic lymphocytic leukemia, and Myeloid panels, highlighting improvements in performance and sensitivity due to new biochemistry and panel design. The process from cell preparation to sequence-ready libraries is reported to be completed within two days. The report emphasizes the platform's ability to detect rare subclones and the robustness of both off-the-shelf and custom panels that can analyze multiple DNA targets simultaneously. Additionally, it discusses the workflow and data completeness achieved with the panels, focusing on the high panel uniformity and the sensitivity demonstrated through various tests. The document serves as a comprehensive resource on the capabilities and enhancements of single-cell DNA sequencing technology.