AI-Enabled Automation of 3D Organoid Culture Processes

This technical report outlines the development and functionality of the CellXpress.ai Automated Cell Culture System, designed to facilitate the fully automated culture of 3D organoids. The report details the system's four essential components: a liquid handler, an incubator, an imager, and integrated AI-powered software. It explains the automation of various workflows for three organoid types: mouse intestinal organoids, human intestinal organoids, and patient-derived colorectal tumor organoids. The processes include plating organoid domes, media changes, monitoring via imaging, and passaging based on AI-driven image analysis. The report describes how organoids are imaged every 12 hours and classified into mature or immature phenotypes using machine learning. The AI model triggers automated passaging based on user-defined criteria, ensuring consistency and reducing the need for human intervention. The findings demonstrate a scalable solution for organoid maintenance and expansion, enhancing efficiency in biological research and drug development.