Onso DNA Library Preparation Procedure

This guide details the procedure for preparing hybrid capture libraries using the Onso DNA Library prep kit and associated components. It outlines the necessary materials, including the Onso DNA library prep kit, Onso fragmentation DNA library prep kit, and various other kits and reagents required for the process. The document specifies the workflow for library preparation, including pre-capture PCR, hybrid capture, and post-capture PCR, while emphasizing compatibility with commercially available hybrid capture kits. It also provides best practices for handling DNA input and reagents, including temperature-sensitive components. The guide includes specific instructions for thermal cycler programs and the number of PCR cycles based on the input DNA quantity. Additionally, it mentions the importance of library size quality control and quantification using recommended sizing technologies. Overall, this document serves as a comprehensive resource for researchers utilizing the Onso system for DNA library preparation.