tRanslatome R/Bioconductor Package Overview

This document is a technical report detailing the tRanslatome package, which is designed for analyzing translational control in gene expression. It provides a comprehensive platform for comparing data from two parallel high-throughput assays, specifically focusing on the translatome and transcriptome. The report outlines the functionalities of the package, including the detection and comparison of differentially expressed genes (DEGs) and the enrichment analysis through Gene Ontology (GO) annotation. It describes the methods available for users to visualize results, such as scatterplots and histograms, and emphasizes the importance of quality control in data analysis. The document also includes practical examples of how to implement the package using R code, showcasing its application in analyzing gene expression data from differentiated and undifferentiated human HepaRG cells. The report serves as a guide for researchers looking to utilize the tRanslatome package for their studies in molecular biology.