Mild pH Elution Benefits of Praesto Jetted A50 HipH

This application note presents a study on the performance of Praesto Jetted A50 HipH in the purification of therapeutic antibodies, particularly focusing on its elution capabilities at higher pH levels. The study compares the Praesto Jetted A50 HipH resin with three other Protein A resins, assessing their effectiveness in terms of antibody purity and residual host cell protein (HCP) levels. The investigation involved various antibody types, including Fc-fusion proteins, monoclonal antibodies (mAbs), and bispecific antibodies. Results indicated that while standard Protein A resins eluted at pH 3.5, the Praesto Jetted A50 HipH allowed elution at pH levels between 4.5 and 4.8, which significantly reduced aggregation and improved yield for bispecific antibodies. The findings demonstrate that Praesto Jetted A50 HipH not only enhances the yield of biologically active monomers but also reduces residual HCP, thereby optimizing the purification process and improving overall process economics.