Comparison of Protein Sequencing Analysis on Platinum and Mass Spectrometry

This technical report presents a comparison of protein sequencing methodologies, specifically focusing on the analysis of the proteins CDNF, IL6, and FGF2 using Quantum-Si's Platinum® platform and traditional mass spectrometry (MS). The report outlines the advancements in proteomics facilitated by mass spectrometry, detailing the typical workflow, including protein digestion and analysis stages. It highlights the challenges faced in MS, such as missed cleavages and the complexity of the workflow, which often necessitates advanced expertise. The Platinum® technology is introduced as a benchtop solution that simplifies the sequencing process, allowing for quicker results without the need for extensive equipment or expertise. The methodology section describes the specific techniques used for sequencing on Platinum, including the preparation of peptide libraries and the use of dye-tagged N-terminal amino acid recognizers. Results indicate that both methods successfully identify the CDNF protein, although they differ in the peptides identified, demonstrating the efficacy of the Platinum® platform in providing a streamlined approach to protein sequencing.