Identifying Monoclonal Antibodies with Quantum-Si Technology

This application note presents a method for identifying low-abundance monoclonal antibodies using Quantum-Si’s next-generation protein sequencing technology on the Platinum instrument. The document outlines the significance of detecting low-abundance monoclonal antibodies in various research and clinical settings, including disease diagnostics and vaccine development. It discusses existing methodologies that face challenges such as high costs and complex procedures, which limit their application. The application note details the methodology employed to sequence Fab fragments of two monoclonal antibodies mixed with polyclonal antibodies, demonstrating the effectiveness of the Platinum platform in identifying unique peptide sequences. The results indicate that the technology can successfully detect monoclonal antibodies even at low abundance levels, thereby addressing traditional challenges associated with antibody identification. The findings suggest that Quantum-Si’s technology has the potential to enhance research in immunology, disease diagnostics, and therapeutic development, contributing to advancements in precision medicine.