Impact of Low pH Affinity Chromatography Elution on AAV Transduction

This white paper presents an analysis of the effects of low pH elution from affinity chromatography on the transduction efficiency and stability of adeno-associated virus (AAV) serotypes 5, 8, and 9. The study investigates various chromatography elution conditions, including pH levels ranging from 2 to 4.5, and their impact on capsid stability and infectivity. The research utilizes size exclusion chromatography coupled with multi-angle light scattering and cell-based transduction assays to assess virus stability and efficiency. Findings indicate that incubation at 25°C for up to 4 hours does not significantly affect capsid stability or infectivity for all serotypes. However, incubation at 40°C resulted in aggregation and loss of infectivity specifically for AAV8. The paper emphasizes the importance of understanding low pH elution effects to optimize vector purification strategies for gene therapy applications, ensuring the production of stable and effective viral vectors.