Guidelines for Analyzing GPCR Second Messenger Data

This white paper presents guidelines and recommendations for analyzing and interpreting GPCR second messenger data. It addresses the ongoing debates regarding methods for analyte quantification, specifically focusing on the communication gap among researchers from various fields such as high-throughput screening, pharmacology, and medicinal chemistry. The document summarizes the outcomes of a workshop held by Revvity with a focus group of experts in GPCR biology and lead discovery. The aim was to establish a common understanding and provide methodological recommendations for biologists, screeners, pharmacologists, and medicinal chemists. The paper outlines four methods for data analysis, detailing how to plot and interpret results from experiments involving second messenger quantification. Additionally, it includes a case study on the M1 receptor, demonstrating how different agonists can be analyzed using the proposed methods. The paper emphasizes the importance of selecting appropriate analysis techniques for effective compound screening.