Automation of KAPA EvoPrep Kit for NGS Library Preparation

This technical report details the automation of Roche’s KAPA EvoPrep Kit for next-generation sequencing (NGS) library preparation on the Beckman Coulter Life Sciences Biomek i7 Hybrid Workstation. The report outlines the methods used for library preparation, including the processing of human genomic DNA (gDNA) with varying input amounts. It documents the performance of automated workflows compared to manual processing, highlighting that the automated method produces consistent yields and fragment sizes across a wide range of input amounts. The study emphasizes the efficiency of the KAPA EvoPrep Kit in handling challenging sample types, such as cell-free DNA and DNA from formalin-fixed paraffin-embedded tissue. The results indicate that the automation improves reproducibility and throughput, making it suitable for high-throughput library construction. The report concludes that the automated workflow provides reliable and efficient library preparation, which is crucial for advancing NGS applications in research settings.