BioPAT Spectro for Raman Calibration Model Transfer

This technical report discusses the implementation of Sartorius' BioPAT® Spectro in the context of Raman spectroscopy for biopharmaceutical manufacturing. It outlines the challenges associated with developing calibration models for Raman-based monitoring and control, particularly the need for model transferability across different bioreactor scales. The report details how the BioPAT® Spectro addresses these challenges by utilizing an identical flow cell design for both small-scale (Ambr®) and larger-scale (Biostat STR®) bioreactors. It presents findings from experiments that demonstrate the successful transfer of Raman calibration models developed in small-scale bioreactors to larger systems, ensuring consistent prediction accuracy. The report further explains the methodology used for generating Raman calibration data, including the use of multivariate data analysis and the integration of various analytical systems. Results indicate that the calibration models maintain high correlation coefficients, confirming the efficacy of the BioPAT® Spectro system for robust data generation in bioprocess applications.