Multiplexing on Unisart StructSure Membranes for Sepsis Detection

This white paper presents advancements in multiplex lateral flow assays (LFA) utilizing Unisart StructSure membranes for the detection of sepsis biomarkers, specifically C-reactive protein (CRP) and Procalcitonin (PCT). The document outlines the limitations of traditional lateral flow tests, such as false negatives and high antibody consumption, and introduces a novel approach that miniaturizes test lines to spots, significantly enhancing assay performance. The paper details the advantages of dot-based LFAs, including reduced antibody usage and improved accuracy through better fluid dynamics. It explains how the unique structured membranes facilitate laminar flow, which is crucial for effective analyte interaction and assay reliability. The white paper also discusses the critical role of early and accurate diagnosis in sepsis management, highlighting how the combination of CRP and PCT biomarkers can provide a more comprehensive assessment of patient condition. The methodology for creating multiplex assays and the potential for quantitative evaluation using advanced readout systems are also described.