Phenotypic and Functional Characterization of CAR-T Cells

This white paper discusses the phenotypic and functional characterization of chimeric antigen receptor T (CAR-T) cells through high-throughput screening and live-cell analysis. It outlines the advancements in immunotherapy, particularly the use of CAR-T cells in targeting cancer cells while minimizing damage to healthy cells. The document details the process of manufacturing and expanding CAR-T cells, emphasizing the importance of quality assessment at each stage. It presents in vitro assays used to evaluate the efficacy and safety of CAR-T cells, including flow cytometry for phenotyping and functional assays for tumor killing. Additionally, the paper highlights the significance of maintaining desirable T cell phenotypes during expansion, such as central memory T cells, which are crucial for sustained anti-tumor responses. Case studies demonstrate the application of the iQue® High-Throughput Screening Platform and Incucyte® Live-Cell Analysis System in profiling CAR-T cells, showcasing their utility in enhancing the understanding of CAR-T cell functionality.