Effect of CZE Buffer Components on Nanobody Separation

This technical note explores the optimization of capillary zone electrophoresis (CZE) buffer components to improve the separation of charge variants in Nanobody samples. It evaluates the SCIEX CZE kit for reproducibility and reduced variability in testing. Key findings include the impact of buffer pH and composition on assay performance, highlighting the importance of product quality attributes in biopharmaceuticals.