Development of GDF15-GFRAL Drug Discovery Assays

This case study outlines the development of a multimodal assay system for GDF15/GFRAL drug discovery. It details the significance of the GDF15-GFRAL-RET signaling complex in research, particularly its role in cellular stress response and energy balance. The document describes the methodologies employed in custom cell line generation, including the use of advanced technology for high-quality reagents and the development of tailored assays. High-throughput screening capabilities in 96- or 384-well formats are highlighted for rapid hit identification. The study presents data from pERK ELISA and SRE reporter assays, comparing their effectiveness in measuring GDF15-induced responses. Results indicate that both assays yield comparable EC and IC50 values, confirming the SRE reporter assay as a viable option for screening. The case study emphasizes the integration of custom cell lines into existing workflows to optimize reproducibility and data reliability, supporting drug discovery initiatives.