Characterization of Biomacromolecules by FPLC coupled with LenS3 MALS Detector

This application note presents a technical report on the use of the LenS3 Multi-Angle Light-Scattering (MALS) detector in conjunction with the ÄKTA pure FPLC system for the characterization of biomacromolecules, specifically monoclonal antibodies (mAbs) and bovine serum albumin (BSA). The document outlines the methodology employed, including the use of size-exclusion chromatography (SEC) for the separation of biomaterials, and details the conditions under which the experiments were conducted. Results indicate that the LenS3 detector provides reliable molecular weight determinations for both mAbs and BSA, even at low concentrations of impurities. The report emphasizes the system's sensitivity and reproducibility, highlighting its capability to accurately calculate molecular weights independent of retention time shifts. Additionally, it discusses the detection linearity and the robustness of the measurements, providing a comprehensive understanding of the performance of the LenS3 MALS detector in protein characterization applications.