High-Resolution DAR Profiling Using TSKgel HIC-ADC Butyl

This application note presents a technical report on the performance of the TSKgel HIC-ADC Butyl column for the analysis of antibody-drug conjugates (ADCs). The study outlines the challenges associated with the characterization of high drug-to-antibody ratio (DAR) species, particularly in terms of resolution and reproducibility. The TSKgel HIC-ADC Butyl column is compared against a conventional reference column, demonstrating superior separation capabilities for various ADCs with heterogeneous payloads. The report details experimental conditions, including the mobile phase composition and gradient used during analysis. Results indicate that the TSKgel HIC-ADC Butyl column significantly improves DAR calculation robustness and reproducibility, particularly for high DAR species. The findings suggest that this column is a reliable tool for ADC characterization, enhancing the accuracy of average DAR determinations. Additionally, the report includes performance parameters and chromatographic data, supporting the column's suitability for regulatory compliance and quality control in biopharmaceutical applications.