Real-Time Monitoring in Shake Flask Cultivations

This technical report documents a study evaluating the use of a Multiparameter Sensor (MPS) for continuous monitoring of biomass and dissolved oxygen in shake flasks during microbial cultivation processes. Shake flasks are commonly used in early-stage bioprocess development due to their simplicity and cost-effectiveness; however, they often lack reliable online monitoring tools. The study highlights the critical role of oxygen availability for the growth of Escherichia coli, a key microorganism in biotechnology. The findings indicate that inadequate oxygen supply can lead to significant limitations in microbial growth and product yield. By implementing the MPS, researchers were able to obtain real-time insights into microbial dynamics, which facilitated the optimization of key process parameters such as agitation speed and fill volume. The results demonstrated a nearly 2.5-fold increase in biomass concentration, showcasing the potential for enhanced scalability and performance in bioprocess development. The report emphasizes the importance of continuous monitoring in improving bioprocess outcomes.