Evaluation of HPLC Systems for Monoclonal Antibody Analysis

This case study evaluates the performance of the Alliance iS Bio HPLC System against legacy systems for size-exclusion chromatography in analyzing monoclonal antibody drugs. It highlights the advantages of reduced system dispersion and improved chromatographic quality, addressing challenges like peak tailing and non-specific adsorption. The findings demonstrate the effectiveness of modern biocompatible systems in ensuring accurate measurements of aggregation and fragmentation in biologics.