Troubleshooting Peak Splitting in HILIC Method Migration

This case study explores the challenges of migrating Hydrophilic Interaction Liquid Chromatography (HILIC) methods, specifically addressing peak splitting issues observed during the analysis of Cetirizine hydrochloride. It highlights the importance of needle wash composition and HPLC system design in achieving reliable chromatographic results. The study compares the Alliance iS HPLC System and Vendor X HPLC System to identify optimal conditions for method migration and troubleshooting.