This guide outlines best practices for the development and operation of weigh stations, which are essential for enforcing vehicle size and weight regulations as mandated by the US Code of Federal Regulations Title 23. It describes the roles of fixed inspection stations and mobile or virtual inspection stations in ensuring compliance and enhancing roadway safety. The document details the necessary infrastructure, technology, and staffing requirements for effective weigh station operations. It emphasizes the importance of utilizing technology for vehicle screening and data management, which includes features such as roadside devices for initial compliance checks and secondary screening opportunities. Additionally, the guide presents considerations for site selection, asset management, and the establishment of policies and procedures that support the efficient functioning of weigh stations. Overall, it serves as a comprehensive resource for state departments of transportation and law enforcement agencies involved in commercial vehicle regulation.